• Product NameBeta-Actin Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.A2-F6
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic peptide (KLH-coupled) within human Beta-actin N terminal.
• Alternative Names    A26C1A antibody
  A26C1B antibody
  ACTB antibody
  ACTB_HUMAN antibody
  Actin beta antibody
  Actin cytoplasmic 1 antibody
  Actin, cytoplasmic 1, N-terminally processed antibody
  Actx antibody
  b actin antibody
  Beta cytoskeletal actin antibody
  Beta-actin antibody
  BRWS1 antibody
  E430023M04Rik antibody
  MGC128179 antibody
  PS1TP5 binding protein 1 antibody
  PS1TP5BP1 antibody
  
• Accession No. Swiss-Prot#:P60709
• UniprotP60709
• Gene ID60;
• Calculated MW 42kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:10,000
  IHC: 1:100-1:200
  ICC: 1:100-1:200
  
  FC: 1:100-1:200

Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.

• Western blot analysis of Beta-Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution.
  Positive control:
  Lane 1: NIH/3T3
  Lane 2: PC12
  Lane 3: MCF-7
  Lane 4: HepG2
  Lane 5: Hela
  Lane 6: Mouse lung

• Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
• ICC staining of Beta-actin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining of Beta-actin in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining of Beta-actin in NIH-3T3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela?cells with Beta-actin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting