• Product NameHER2 / ErbB2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPeptide affinity purified
• ApplicationsWB, ICC, IHC
• Species ReactivityHu
• Immunogen Descriptionpeptide
• Alternative Names    Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
  C erb B2/neu protein antibody
  CD340 antibody
  CD340 antigen antibody
  Cerb B2/neu protein antibody
  CerbB2 antibody
  Erb b2 receptor tyrosine kinase 2 antibody
  ERBB2 antibody
  ERBB2_HUMAN antibody
  HER 2 antibody
  HER 2/NEU antibody
  HER2 antibody
  Herstatin antibody
  Human epidermal growth factor receptor 2 antibody
  Metastatic lymph node gene 19 protein antibody
  MLN 19 antibody
  MLN19 antibody
  NEU antibody
  NEU proto oncogene antibody
  Neuro/glioblastoma derived oncogene homolog antibody
  Neuroblastoma/glioblastoma derived oncogene homolog antibody
  NGL antibody
  p185erbB2 antibody
  Proto-oncogene c-ErbB-2 antibody
  Proto-oncogene Neu antibody
  Receptor tyrosine-protein kinase erbB-2 antibody
  TKR1 antibody
  Tyrosine kinase type cell surface receptor HER2 antibody
  Tyrosine kinase-type cell surface receptor HER2 antibody
  V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
  V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
  V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
  V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
  V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
  Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
  
• Accession No. Swiss-Prot#:P04626
• UniprotP04626
• Gene ID2064;
• Calculated MW 140kDa
• Formulation 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:200
  ICC: 1:200
  
  

HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. Amplification or overexpression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years the protein has become an important biomarker and target of therapy for approx. 30% of breast cancer patients. Over-expression is also known to occur in ovarian, stomach, and aggressive forms of uterine cancer, such as uterine serous endometrial carcinoma. The expression of HER2 is regulated by signaling through estrogen receptors. Normally, estradiol and tamoxifen acting through the estrogen receptor down-regulate the expression of HER2. However, when the ratio of the coactivator AIB-3 exceeds that of the corepressor PAX2, the expression of HER2 is upregulated in the presence of tamoxifen, leading to tamoxifen-resistant breast cancer.

• Western blot analysis of ErbB2 on different cell lysates using anti- ErbB2 antibody at 1/500 dilution. Positive control:
  Lane 1: SKOV-3
  Lane2: SKBR-3
• Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Her 2 antibody. Counter stained with hematoxylin.
• ICC staining ErbB2 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting