• Product NameCacng4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPeptide affinity purified.
• ApplicationsWB,ICC,IHC,FC
• Species ReactivityHu, Rt
• Immunogen DescriptionSynthetic peptide within rat Cacng4 aa 150-250.
• Alternative Names    CACNG4 antibody
  Calcium channel voltage dependent gamma subunit 4 antibody
  CCG4_HUMAN antibody
  Neuronal voltage gated calcium channel gamma 4 subunit antibody
  Neuronal voltage-gated calcium channel gamma-4 subunit antibody
  TARP gamma-4 antibody
  Transmembrane AMPAR regulatory protein gamma-4 antibody
  Voltage dependent calcium channel gamma 4 subunit antibody
  Voltage-dependent calcium channel gamma-4 subunit antibody
  
• Accession No. Swiss-Prot#:Q9UBN1
• UniprotQ9UBN1
• Gene ID27092;
• Calculated MW 37 kDa
• Formulation 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50
  ICC: 1:50-1:100
  
  FC: 1:50-1:100

Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit. Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs), including GRIA1 and GRIA4. Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization.

• Western blot analysis of Cacng4 on MCF-7 cell lysate using anti-Cacng4 antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.
• ICC staining Cacng4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Cacng4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SH-SY5Y cells with Cacng4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting