• Product NameCaspase-3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPeptide affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu, Ms
• Immunogen DescriptionSynthetic peptide (KLH-coupled) witihin human Caspase-3 N-terminal.
• Alternative Names    A830040C14Rik antibody
  Apopain antibody
  CASP-3 antibody
  CASP3 antibody
  CASP3_HUMAN antibody
  Casp3a antibody
  Caspase 3 antibody
  Caspase 3, apoptosis-related cysteine peptidase antibody
  Caspase 3, apoptosis-related cysteine protease antibody
  Caspase 3, apoptosis-related cysteine protease a antibody
  Caspase-3 subunit p12 antibody
  CC3 antibody
  CPP-32 antibody
  CPP32 antibody
  CPP32B antibody
  Cysteine protease CPP32 antibody
  EC 3.4.22.56 antibody
  LICE antibody
  mldy antibody
  OTTHUMP00000165052 antibody
  OTTHUMP00000165053 antibody
  OTTHUMP00000165054 antibody
  PARP cleavage protease antibody
  Procaspase3 antibody
  Protein Yama antibody
  SCA 1 antibody
  SCA-1 antibody
  SREBP cleavage activity 1 antibody
  Yama antibody
  
• Accession No. Swiss-Prot#:P42574
• UniprotP42574
• Gene ID836;
• Calculated MW 31 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:200
  ICC: 1:200
  
  FC: 1:100-1:200

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.

• Western blot analysis of Caspase-3 on different lysates using anti- Caspase-3 antibody at 1/1,000 dilution.
  Positive control:  
  Lane 1: Human lung   Lane 2: Human brain  
  Lane 3: Jurkat         Lane 4: F9  
  Lane 5: NCCTI         Lane 6: Daudi  
  Lane 7: Raji            Lane 8: MCF-7  
  Lane 9: Hela

• Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.
• ICC staining of Caspase-3 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining of Caspase-3 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with Caspase-3 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting