• Product NameCD133 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPeptide affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu
• Immunogen Descriptionrecombinant protein
• Alternative Names    AC133 antibody
  Antigen AC133 antibody
  CD133 antibody
  CORD12 antibody
  Hematopoietic stem cell antigen antibody
  hProminin antibody
  MCDR2 antibody
  MSTP061 antibody
  OTTHUMP00000217744 antibody
  OTTHUMP00000217745 antibody
  OTTHUMP00000217746 antibody
  PROM1 antibody
  PROM1_HUMAN antibody
  Prominin I antibody
  Prominin like 1 antibody
  Prominin like protein 1 precursor antibody
  Prominin mouse like 1 antibody
  Prominin-1 antibody
  Prominin-like protein 1 antibody
  Prominin1 antibody
  PROML1 antibody
  RP41 antibody
  STGD4 antibody
  
• Accession No. Swiss-Prot#:O43490
• UniprotO43490
• Gene ID8842;
• Calculated MW 100 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  IHC: 1:200
  ICC: 1:200
  
  FC: 1:100-1:200

CD133 is a glycoprotein also known in humans and rodents as Prominin 1 (PROM1). It is the founding member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localizes to cellular protrusions. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastomas, neuronal and glial stem cells and some other cell types. CD133 is recognized as a stem cell marker for normal and cancerous tissues.

• Western blot analysis of CD133 on different cell lysates using anti-CD133 antibody at 1/1000 dilution.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD133 antibody. Counter stained with hematoxylin.
• ICC staining CD133 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD133 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD133 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SHG-44 cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
• Flow cytometric analysis of HUVEC cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting