• Product NameJNK1+JNK2+JNK3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SA43-06
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IP
• Species ReactivityHu, Ms, Rt, Cow, Monkey, zebrafish
• Immunogen Descriptionrecombinant protein
• Alternative Names    C Jun kinase 2 antibody
  c Jun N terminal kinase 1 antibody
  c Jun N terminal kinase 2 antibody
  c Jun N terminal kinase 3 antibody
  c-Jun N-terminal kinase 1 antibody
  JNK 46 antibody
  JNK 55 antibody
  JNK antibody
  JNK-46 antibody
  JNK1 antibody
  JNK1A2 antibody
  JNK2 antibody
  JNK21B1/2 antibody
  JNK2A antibody
  JNK2ALPHA antibody
  JNK2B antibody
  JNK2BETA antibody
  JNK3 alpha protein kinase antibody
  JNK3 antibody
  JNK3A antibody
  Jun kinase antibody
  JUN N terminal kinase antibody
  MAP kinase 10 antibody
  MAP kinase 8 antibody
  MAP kinase 9 antibody
  MAP kinase p49 3F12 antibody
  MAPK 10 antibody
  MAPK 8 antibody
  MAPK 9 antibody
  MAPK10 antibody
  mapk8 antibody
  MAPK9 antibody
  Mitogen activated protein kinase 10 antibody
  Mitogen activated protein kinase 8 antibody
  Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
  Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
  Mitogen activated protein kinase 9 antibody
  Mitogen-activated protein kinase 8 antibody
  MK08_HUMAN antibody
  p493F12 antibody
  p54a antibody
  p54aSAPK antibody
  p54bSAPK antibody
  PRKM10 antibody
  PRKM8 antibody
  PRKM9 antibody
  SAPK antibody
  SAPK(beta) antibody
  SAPK1 antibody
  SAPK1a antibody
  SAPK1b antibody
  SAPK1c antibody
  Stress activated protein kinase 1 antibody
  Stress activated protein kinase 1a antibody
  Stress activated protein kinase 1b antibody
  Stress activated protein kinase 1c antibody
  Stress activated protein kinase beta antibody
  Stress activated protein kinase JNK1 antibody
  Stress activated protein kinase JNK2 antibody
  Stress activated protein kinase JNK3 antibody
  Stress-activated protein kinase 1c antibody
  Stress-activated protein kinase JNK1 antibody
  
• Accession No. Swiss-Prot#:P45983
• UniprotP45983
• Gene ID5599;
• Calculated MW 46/54 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  
  ICC: 1:50-1:200
  
  

c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38.

• Western blot analysis of JNK1+JNK2+JNK3 on different cell lysates using anti-JNK1+JNK2+JNK3 antibody at 1/1,000 dilution. Positive control:
  Lane 1: Hela
  Lane 2: PC12
  Lane 3: K562
  Lane 5: HepG2
  Lane 6: Human lung
• Western blot analysis of JNK1+JNK2+JNK3 on hybrid fish (crucian-carp) brain tissue lysate using anti-JNK1+JNK2+JNK3 antibody at 1/500 dilution.

• ICC staining JNK1+JNK2+JNK3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting