• Product NameCD9 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SA35-08
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    Tetraspanin 29 antibody
  5H9 antibody
  5H9 antigen antibody
  Antigen defined by monoclonal antibody
  602 29 antibody
  Antigen defined by monoclonal antibody
  60229 antibody
  BA-2/p24 antigen antibody
  BA2 antibody
  BTCC 1 antibody
  BTCC1 antibody
  CD9 antibody
  CD9 antigen antibody
  CD9 antigen p24 antibody
  CD9 molecule antibody
  CD9_HUMAN antibody
  Cell growth inhibiting gene 2 protein antibody
  Cell growth-inhibiting gene 2 protein antibody
  DRAP 27 antibody
  DRAP27 antibody
  GIG2 antibody
  Growth inhibiting gene 2 protein antibody
  Leukocyte antigen MIC3 antibody
  MIC3 antibody
  Motility related protein antibody
  Motility-related protein antibody
  MRP 1 antibody
  MRP-1 antibody
  MRP1 antibody
  p24 antibody
  p24 antigen antibody
  Tetraspanin-29 antibody
  Tspan 29 antibody
  Tspan-29 antibody
  TSPAN29 antibody
  
• Accession No. Swiss-Prot#:P21926
• UniprotP21926
• Gene ID928;
• Calculated MW 25 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:20-1:50

CD9 is a type IV transmembrane glycoprotein with four transmembrane domains. CD9 on pre-B cells may play a role in cell-cell adhesion. In addition, CD9 may play a role in signal transduction mediated by interaction with low molecular weight GTP-binding proteins. CD9 is expressed on early B cells, eosinophils, basophils and activated T cells and is a major component of the platelet cell surface. It is also expressed on most non-T acute lymphoblastic leukemia cells and on some acute myeloid and chronic lymphoid leukemias.

• Western blot analysis of CD9 on different lysates using anti-CD9 antibody at 1/500 dilution. Positive control:
  Lane 1: Mouse heart
  Lane 2: Jurkat
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD9 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD9 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.
• ICC staining CD9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD9 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting