• Product NameMEK1/2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SR13-07
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP
• Species ReactivityHu, Ms, Rt, zebrafish
• Immunogen Descriptionrecombinant protein
• Alternative Names    AA589381 antibody
  CFC3 antibody
  Dual specificity mitogen-activated protein kinase kinase 1 antibody
  Dual specificity mitogen-activated protein kinase kinase 2 antibody
  EC 2.7.12.2 antibody
  ERK activator kinase 1 antibody
  ERK activator kinase 2 antibody
  FLJ26075 antibody
  MAP kinase kinase 1 antibody
  MAP kinase kinase 2 antibody
  MAP2K1 antibody
  MAP2K2 antibody
  MAPK/ERK kinase 1 antibody
  MAPK/ERK kinase 2 antibody
  MAPKK 1 antibody
  MAPKK1 antibody
  MAPKK2 antibody
  MEK 1 antibody
  MEK1 antibody
  MEKK1 antibody
  Mitogen activated protein kinase kinase 1 antibody
  Mitogen activated protein kinase kinase 2 antibody
  Mitogen-activated protein kinase kinase 2, p45 antibody
  MK2 antibody
  MKK 1 antibody
  MKK 2 antibody
  MKK1 antibody
  MKK2 antibody
  MP2K1_HUMAN antibody
  PRKMK 1 antibody
  PRKMK 2 antibody
  Prkmk1 antibody
  Prkmk2 antibody
  protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody
  Protein kinase, mitogen-activated, kinase 1 antibody
  Protein kinase, mitogen-activated, kinase 2 antibody
  
• Accession No. Swiss-Prot#:P36507
• UniprotP36507
• Gene ID5605;
• Calculated MW 44 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

• Western blot analysis of MEK1/2 on different cell lysates using anti-MEK1/2 antibody at 1/1,000 dilution. Positive control:
  Lane 1: HepG2
  Lane 2: PC12
  Lane 3: NIH/3T3
• Western blot analysis of MEK1/2 on hybrid fish (crucian-carp) brain tissue lysates using anti-MEK1/2 antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.
• ICC staining MEK1/2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining MEK1/2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting