• Product NameFirefly Luciferase Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SR03-86
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF
• Species ReactivityFirefly
• Immunogen DescriptionRecombinant protein
• Alternative Names    ec 1.13.12.7 antibody
  Firefly antibody
  Luciferase antibody
  Luciferin 4 monooxygenase antibody
  
• Accession No. Swiss-Prot#:P08659
• UniprotP08659
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  
  
  
  

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

• Western blot analysis of Firefly Luciferase on luciferase recombinant protein lysate using anti-Firefly Luciferase antibody at 1/1,000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting