• Product Namep16 ARC Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SR34-02
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    Actin related protein 2/3 complex subunit 5 (16 kD) antibody
  Actin related protein 2/3 complex subunit 5 antibody
  Actin related protein 2/3 complex, subunit 5 16kDa antibody
  Actin-related protein 2/3 complex subunit 5 antibody
  ARC16 antibody
  Arp2/3 complex 16 kDa subunit antibody
  Arp2/3 protein complex subunit p16 antibody
  ARPC 5 antibody
  Arpc5 antibody
  ARPC5_HUMAN antibody
  dJ127C7.3 antibody
  MGC88523 antibody
  p16 Arc antibody
  p16-ARC antibody
  RP1 127C7.3 antibody
  
• Accession No. Swiss-Prot#:O15511
• UniprotO15511
• Gene ID10092;
• Calculated MW 16 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50
  
  FC: 1:50-1:100

The Arp2/3 (Actin-related protein 2/3) complex consists of seven subunits, all of which are actin-related proteins. The complex is involved in the control of actin polymerization and in mediating the formation of branched actin networks. p16-ARC, also known as ARPC5 (Actin-related protein 2/3 complex subunit 5) or ARC16 (Arp2/3 complex 16 kDa subunit), is a 151 amino acid subunit of the Arp2/3 complex. Thought to play a role in maintaining the integrity of Arp2/3, p16-ARC is a substrate for MAPKAPK-2 which, through phosphorylation of p16-ARC, may participate in Arp2/3 regulatory functions and remodeling of the Actin cytoskeleton. Two isoforms of p16-ARC exist due to alternative splicing events.

• Western blot analysis of p16 ARC on different cell lysates using anti-p16 ARC antibody at 1/1,000 dilution. Positive control:
  Lane 1: MCF-7
  Lane 2: SK-BR-3
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.
• ICC staining p16 ARC in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SH-SY-5Y cells with p16 ARC antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting