• Product NameAIF Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SZ05-01
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, IP, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    AIFM1 antibody
  AIFM1_HUMAN antibody
  Apoptosis inducing factor 1, mitochondrial antibody
  Apoptosis inducing factor antibody
  Apoptosis inducing factor, mitochondrion associated, 1 antibody
  Apoptosis-inducing factor 1 antibody
  CMTX4 antibody
  COWCK antibody
  COXPD6 antibody
  Harlequin antibody
  Hq antibody
  mAIF antibody
  MGC111425 antibody
  MGC5706 antibody
  mitochondrial antibody
  Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody
  neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody
  PDCD 8 antibody
  PDCD8 antibody
  Programmed cell death 8 (apoptosis inducing factor) antibody
  Programmed cell death 8 antibody
  Programmed cell death 8 isoform 1 antibody
  Programmed cell death 8 isoform 2 antibody
  Programmed cell death 8 isoform 3 antibody
  Programmed cell death protein 8 antibody
  Programmed cell death protein 8 mitochondrial antibody
  Programmed cell death protein 8 mitochondrial precursor antibody
  Programmed cell death protein 8 mitochondrial precursor antibody
  Striatal apoptosis inducing factor antibody
  
• Accession No. Swiss-Prot#:O95831
• UniprotO95831
• Gene ID9131;
• Calculated MW 67 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity.

• Western blot analysis of AIF on different lysates using anti-AIF antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: SKOV-3

• Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AIF antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AIF antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-AIF antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AIF antibody. Counter stained with hematoxylin.
• ICC staining AIF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining AIF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with AIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting