• Product NamePI 3 Kinase catalytic subunit alpha Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SJ0186
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IP
• Species ReactivityHu, Ms
• Immunogen Descriptionrecombinant protein
• Alternative Names    5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody
  5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody
  caPI3K antibody
  CLOVE antibody
  CWS5 antibody
  MCAP antibody
  MCM antibody
  MCMTC antibody
  MGC142161 antibody
  MGC142163 antibody
  p110 alpha antibody
  p110alpha antibody
  Phosphatidylinositol 3 kinase catalytic alpha polypeptide antibody
  Phosphatidylinositol 3 kinase catalytic 110 KD alpha antibody
  Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha antibody
  Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform antibody
  Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha antibody
  Phosphatidylinositol-4 antibody
  Phosphoinositide 3 kinase catalytic alpha polypeptide antibody
  PI3 kinase p110 subunit alpha antibody
  PI3-kinase subunit alpha antibody
  PI3K antibody
  PI3K-alpha antibody
  PI3KC A antibody
  PIK3C A antibody
  Pik3ca antibody
  PK3CA antibody
  PK3CA_HUMAN antibody
  PtdIns 3 kinase p110 antibody
  PtdIns-3-kinase subunit alpha antibody
  PtdIns-3-kinase subunit p110-alpha antibody
  Serine/threonine protein kinase PIK3CA antibody
  
• Accession No. Swiss-Prot#:P42336
• UniprotP42336
• Gene ID5290;
• Calculated MW 124 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1000
  
  ICC: 1:50-1:200
  
  

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins.

• ICC staining PI 3 kinase p110 alpha in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PI 3 kinase p110 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PI 3 kinase p110 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting