• Product NamePARP Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SU03-68
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
  ADP ribosyltransferase diphtheria toxin like 1 antibody
  ADP ribosyltransferase NAD(+) antibody
  ADPRT 1 antibody
  ADPRT antibody
  ADPRT1 antibody
  ARTD1 antibody
  msPARP antibody
  NAD(+) ADP ribosyltransferase 1 antibody
  NAD(+) ADP-ribosyltransferase 1 antibody
  pADPRT 1 antibody
  pADPRT1 antibody
  PARP 1 antibody
  PARP antibody
  PARP-1 antibody
  PARP1 antibody
  PARP1_HUMAN antibody
  Poly (ADP ribose) polymerase 1 antibody
  poly (ADP ribose) polymerase family, member 1 antibody
  Poly [ADP-ribose] polymerase 1 antibody
  Poly(ADP ribose) polymerase antibody
  poly(ADP ribose) synthetase antibody
  poly(ADP ribosyl)transferase antibody
  Poly[ADP ribose] synthetase 1 antibody
  Poly[ADP-ribose] synthase 1 antibody
  PPOL antibody
  
• Accession No. Swiss-Prot#:P09874
• UniprotP09874
• Gene ID142;
• Calculated MW 113/89 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

• Western blot analysis of PARP on different lysates using anti-PARP antibody at 1/1,000 dilution. Positive control:
  Lane 1: A549
  Lane 2: Jurkat
  Lane 3: Hela
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PARP antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PARP antibody. Counter stained with hematoxylin.
• ICC staining PARP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting