• Product NamePhospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.SU03-70
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic phospho-peptide corresponding to residues surrounding Ser144 of human PAK1.
• Accession No. Swiss-Prot#:O75914
• UniprotO75914
• Gene ID5063;
• Calculated MW 65 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Cytoplasm, Cell membrane.

• Western blot analysis of Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) on different lysates using anti-Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/1,000 dilution. Positive control:
  Lane 1: Hela
  Lane 2: NIH/3T3
  Lane 3: SH-SY-5Y
• Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody. Counter stained with hematoxylin.
• ICC staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of NIH/3T3 cells with Phospho- PAK1(S144)+PAK2(S141)+PAK3(S139) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting