• Product NamePI 3 Kinase p85 beta Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.ST04-77
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    p85 antibody
  p85 beta antibody
  P85B antibody
  P85B_HUMAN antibody
  Phosphatidylinositol 3 kinase antibody
  Phosphatidylinositol 3 kinase regulatory beta subunit antibody
  Phosphatidylinositol 3 kinase regulatory subunit beta antibody
  Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody
  Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta) antibody
  Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody
  Phosphatidylinositol 3-kinase regulatory subunit beta antibody
  Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody
  Phosphoinositide 3 kinase regulatory subunit 2 antibody
  Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody
  Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody
  Phosphoinositide 3 kinase, regulatory subunit 2 (beta) antibody
  Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta) antibody
  PI3 kinase p85 beta subunit antibody
  PI3 kinase p85 subunit beta antibody
  PI3-kinase regulatory subunit beta antibody
  PI3-kinase subunit p85-beta antibody
  PI3K antibody
  PI3K regulatory subunit beta antibody
  PIK3R 2 antibody
  PIK3R2 antibody
  PtdIns 3 kinase p85 beta antibody
  PtdIns-3-kinase regulatory subunit beta antibody
  PtdIns-3-kinase regulatory subunit p85-beta antibody
  
• Accession No. Swiss-Prot#:O00459
• UniprotO00459
• Gene ID5296;
• Calculated MW 82 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110α expression is restricted to white blood cells. It has been shown to bind p85α and p85β, but it apparently does not phosphorylate these subunits. p110α seems to have the capacity to autophosphorylate. p110α does not interact with the p85 subunits. It has been shown to be activated by α and β heterotrimeric G proteins.

• Western blot analysis of PI 3 Kinase p85 beta on different lysates using anti-PI 3 Kinase p85 beta antibody at 1/1,000 dilution. Positive control:
  Lane 1: Raji
  Lane 2: Hela
  Lane 3: MCF-7
  Lane 4: U937
• Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat colon tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.
• ICC staining PI 3 Kinase p85 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PI 3 Kinase p85 beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with PI 3 Kinase p85 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting