• Product NameMetabotropic glutamate receptor 5 Rabbit mAb
• Clone No.ST51-00
• PurificationAffinity-chromatography
• ApplicationsWB;IHC;ICC/IF;FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionA synthesized peptide derived from human mGluR5
• Alternative Names    Glutamate receptor metabotropic 5 antibody
  GPRC1E antibody
  Grm5 antibody
  GRM5_HUMAN antibody
  Metabotropic glutamate receptor 5 antibody
  Metabotropic glutamate receptor 5 variant F antibody
  Metabotropic glutamate receptor 5 variant G antibody
  Metabotropic glutamate receptor 5 variant H antibody
  mGlu5 antibody
  mGluR5 antibody
  PPP1R86 antibody
  Protein phosphatase 1 regulatory subunit 86 antibody
  
• Accession No. Swiss-Prot#:P41594
• UniprotP41594
• Gene ID2915;
• Calculated MW 132 kDa
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

• WB 1:1000-1:2000
  IHC 1:100-1:200
  ICC/IF 1:50-1:200
  FC 1:20-1:100

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.

• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin.
• ICC staining GRM5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining GRM5 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining GRM5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SH-SY-5Y cells with GRM5 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting