• Product NameATP citrate lyase Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.ST51-07
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    ACL antibody
  Acly antibody
  ACLY_HUMAN antibody
  ATP citrate (pro-S) lyase antibody
  ATP citrate lyase antibody
  ATP citrate synthase antibody
  ATP-citrate (pro-S-)-lyase antibody
  ATP-citrate synthase antibody
  ATPcitrate synthase antibody
  ATPCL antibody
  Citrate cleavage enzyme antibody
  CLATP antibody
  OTTHUMP00000164773 antibody
  
• Accession No. Swiss-Prot#:P53396
• UniprotP53396
• Gene ID47;
• Calculated MW 122 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

ATP-citrate synthase, also designated ATP-citrate lyase or citrate cleavage enzyme, is a cytoplasmic homotetramer belonging to the succinate/malate CoA ligase family. The gene coding for this protein maps against chromosome 17q12-q21. ATP-citrate synthase catalyses the formation of acetyl-CoA and oxaloacetate from citrate and CoA. This product, Acetyl-CoA, is necessary for both fatty acid and cholesterol biosynthesis. ATP citrate-lyase is important in the biosynthesis of acetylcholine in nervous tissue.

• Western blot analysis of ATP citrate lyase on different lysates using anti-ATP citrate lyase antibody at 1/1,000 dilution. Positive control:
  Lane 1: Mouse kidney
  Lane 2: Mouse colon
  Lane 3: MCF-7
• Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ATP citrate lyase citrate lyase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
• ICC staining ATP citrate lyase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ATP citrate lyase in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with ATP citrate lyase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting