• Product NameVitronectin Rabbit mAb
• Clone No.12D1
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    Complement S Protein antibody
  Epibolin antibody
  S Protein antibody
  S-protein antibody
  Serum Spreading Factor antibody
  Serum-spreading factor antibody
  Somatomedin B antibody
  Somatomedin-B antibody
  V75 antibody
  Vitronectin V10 subunit antibody
  Vitronectin V65 subunit antibody
  VN antibody
  VNT antibody
  VTN antibody
  VTNC_HUMAN antibody
  
• Accession No. Swiss-Prot#:P04004
• UniprotP04004
• Gene ID7448;
• Calculated MW 75 kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200

A431, A549, MCF-7, HepG2, mouse liver tissue, mouse skin tissue, human liver tissue, human liver cancer tissue.

• Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.
• ICC staining Vitronectin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Vitronectin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Vitronectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Vitronectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Western blot analysis of Vitronectin expression in human Serum membrane lysate.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting