• Product NameCOX2/Cyclooxygenase 2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SC56-06
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF
• Species ReactivityHu, Ms
• Immunogen Descriptionrecombinant protein
• Alternative Names    COX 2 antibody
  COX-2 antibody
  COX2 antibody
  Cyclooxygenase 2 antibody
  Cyclooxygenase 2b antibody
  Cyclooxygenase antibody
  Cyclooxygenase-2 antibody
  Cyclooxygenase2 antibody
  EC 1.14.99.1 antibody
  fj02a10 antibody
  Glucocorticoid-regulated inflammatory cyclooxygenase antibody
  Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
  GRIPGHS antibody
  hCox 2 antibody
  Macrophage activation-associated marker protein P71/73 antibody
  OTTHUMP00000033524 antibody
  PES-2 antibody
  PGG/HS antibody
  PGH synthase 2 antibody
  PGH2_HUMAN antibody
  PGHS 2 antibody
  PGHS-2 antibody
  PGHS2 antibody
  PHS 2 antibody
  PHS II antibody
  PHS2 antibody
  Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
  Prostaglandin endoperoxide synthase 2 antibody
  Prostaglandin G/H synthase 2 antibody
  Prostaglandin G/H synthase 2 precursor antibody
  Prostaglandin G/H synthase and cyclooxygenase antibody
  Prostaglandin G/H synthase antibody
  Prostaglandin H2 synthase 2 antibody
  prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
  Prostaglandin-endoperoxide synthase 2 antibody
  PTGS2 antibody
  ptgs2a antibody
  TIS10 antibody
  TIS10 protein antibody
  unp1239 antibody
  wu:fj02a10 antibody
  
• Accession No. Swiss-Prot#:P35354
• UniprotP35354
• Gene ID5743;
• Calculated MW 72 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  
  ICC: 1:50-1:200
  
  

Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutively expressed in most tissues and is thought to serve in general housekeeping functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs.

• Western blot analysis of COX2 on different lysates using anti-COX2 antibody at 1/1,000 dilution. Positive control:
  Lane 1: A549
  Lane 2: THP-1
• ICC staining COX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining COX2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining COX2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting