• Product Namep63 Rabbit mAb
• Clone No.SC06-31
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    AIS antibody
  Amplified in squamous cell carcinoma antibody
  B(p51A) antibody
  B(p51B) antibody
  Chronic ulcerative stomatitis protein antibody
  CUSP antibody
  DN p63 alpha 1 antibody
  DNp63 antibody
  EEC3 antibody
  id:ibd3516 antibody
  Keratinocyte transcription factor antibody
  Keratinocyte transcription factor KET antibody
  KET antibody
  LMS antibody
  MGC115972 antibody
  MGC192897 antibody
  NBP antibody
  OFC8 antibody
  OTTHUMP00000209732 antibody
  OTTHUMP00000209733 antibody
  OTTHUMP00000209734 antibody
  OTTHUMP00000209735 antibody
  OTTHUMP00000209737 antibody
  OTTHUMP00000209738 antibody
  OTTHUMP00000209739 antibody
  OTTHUMP00000209740 antibody
  OTTHUMP00000209741 antibody
  OTTHUMP00000209742 antibody
  OTTHUMP00000209743 antibody
  OTTHUMP00000209744 antibody
  p40 antibody
  p51 antibody
  P51/P63 antibody
  p53-related protein p63 antibody
  p53CP antibody
  p63 antibody
  P63_HUMAN antibody
  p73H antibody
  p73L antibody
  RHS antibody
  SHFM4 antibody
  TAp63alpha antibody
  TP53CP antibody
  TP53L antibody
  TP63 antibody
  TP73L antibody
  Transformation related protein 63 antibody
  Transformation-related protein 63 antibody
  Trp53rp1 antibody
  Trp63 antibody
  Tumor protein 63 antibody
  Tumor protein p53-competing protein antibody
  Tumor protein p53-like antibody
  Tumor protein p63 antibody
  Tumor protein p63 deltaN isoform delta antibody
  Tumor protein p73 antibody
  Tumor protein p73-like antibody
  
• Accession No. Swiss-Prot#:Q9H3D4
• UniprotQ9H3D4
• Gene ID8626;
• Calculated MW 55 kDa
• Concentration 1.5 mg/ml
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function. While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors. Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck. The p63 gene contains an alternative transcription initiation site that yields a 40 kDa δNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms.

• Western blot analysis of p63 on human kidney lysates using anti-p63 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p63 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-p63 antibody. Counter stained with hematoxylin.
• ICC staining p63 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of A431 cells with p63 antibody at 1/50 dilution (black) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting