• Product NameWilms Tumor Protein Rabbit mAb
• Clone No.SC06-41
• PurificationAffinity-chromatography
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu, Ms
• Immunogen DescriptionA synthesized peptide derived from human WT1
• Alternative Names    WIT 2 antibody
  WT 1 antibody
  AWT1 antibody
  FWT1 antibody
  GUD antibody
  NPHS4 antibody
  WAGR antibody
  Wilms tumor 1 antibody
  Wilms Tumor antibody
  Wilms tumor protein antibody
  Wilms' tumor gene antibody
  Wilms' tumor protein antibody
  WIT2 antibody
  WT antibody
  WT1 antibody
  WT1_HUMAN antibody
  WT33 antibody
  
• Accession No. Swiss-Prot#:P19544
• UniprotP19544
• Gene ID7490;
• Calculated MW 55 kDa
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

• WB 1:1000-1:2000
  IHC 1:100-1:200
  ICC/IF 1:50-1:200

  FC 1:20-1:1000

Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

• ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.
• Western blot analysis of WT1 expression in K562 cell lysate.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting