• Product NamePRP19 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SC06-53
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    hPso4 antibody
  NMP200 antibody
  Nuclear matrix protein 200 antibody
  Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody
  pre mRNA processing factor 19 antibody
  Pre-mRNA-processing factor 19 antibody
  PRP19 antibody
  PRP19/PSO4 homolog antibody
  PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody
  PRP19_HUMAN antibody
  PRPF19 antibody
  PSO4 antibody
  psoralen 4 antibody
  Senescence evasion factor antibody
  SNEV antibody
  UBOX4 antibody
  
• Accession No. Swiss-Prot#:Q9UMS4
• UniprotQ9UMS4
• Gene ID27339;
• Calculated MW 55 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5' splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.

• Western blot analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
• ICC staining PRP19 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PRP19 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PRP19 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with PRP19 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting