• Product NameAKAP14 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SC0667
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IP
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    A kinase (PRKA) anchor protein 14 antibody
  A kinase anchor protein 14 antibody
  A kinase anchor protein 28 kDa antibody
  A-kinase anchoring protein 28 antibody
  AKAP14 antibody
  AKAP28 antibody
  PRKA14 antibody
  Protein kinase A anchoring protein 14 antibody
  
• Accession No. Swiss-Prot#:Q86UN6
• UniprotQ86UN6
• Gene ID158798;
• Calculated MW 23 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  
  ICC: 1:50-1:200
  
  

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

• Western blot analysis of AKAP14 on different lysates using anti-AKAP14 antibody at 1/1,000 dilution. Positive control:
  Lane 1: Jurkat
  Lane 2: A549
• ICC staining AKAP14 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining AKAP14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining AKAP14 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting