• Product NameNLRP3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SC06-23
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    AGTAVPRL antibody
  AII/AVP antibody
  Angiotensin/vasopressin receptor AII/AVP like antibody
  Angiotensin/vasopressin receptor AII/AVP-like antibody
  C1orf7 antibody
  Caterpiller protein 1.1 antibody
  CIAS 1 antibody
  CIAS1 antibody
  CLR1.1 antibody
  Cold autoinflammatory syndrome 1 antibody
  Cold autoinflammatory syndrome 1 protein antibody
  Cryopyrin antibody
  Familial cold autoinflammatory syndrome antibody
  FCAS antibody
  FCU antibody
  LRR and PYD domains-containing protein 3 antibody
  Muckle-Wells syndrome antibody
  MWS antibody
  NACHT antibody
  NACHT LRR and PYD containing protein 3 antibody
  NALP 3 antibody
  NALP3 antibody
  NALP3_HUMAN antibody
  NLRP3 antibody
  PYPAF 1 antibody
  PYPAF1 antibody
  PYRIN containing APAF1 like protein 1 antibody
  PYRIN-containing APAF1-like protein 1 antibody
  
• Accession No. Swiss-Prot#:Q96P20
• UniprotQ96P20
• Gene ID114548;
• Calculated MW 118/85 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  FC: 1:50-1:100

Cryopyrin interacts selectively with apoptosis-associated specklike protein containing a CARD domain (ASC). This complex may function as an upstream activator of NFkB signaling and caspase-1 activation. The complex also inhibits TNFa induced activation and nuclear translocation of RelA/NFkB p65. Mutations in Cryopyrin and Pyrin proteins are responsible for several autoinflammatory disorders in humans, including familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA).

• Flow cytometric analysis of Jurkat cells with NLRP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
• ICC staining NLRP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NLRP3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting