• Product NameRAD18 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SN66-01
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    2810024C04Rik antibody
  DNA repair protein rad18 antibody
  E3 ubiquitin-protein ligase RAD18 antibody
  EC 6.3.2.- antibody
  hHR 18 antibody
  hHR18 antibody
  hRAD 18 antibody
  hRAD18 antibody
  MGC156682 antibody
  Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody
  Postreplication repair protein hRAD18p antibody
  Postreplication repair protein RAD18 antibody
  RAD 18 antibody
  RAD18 antibody
  RAD18 homolog (S. cerevisiae) antibody
  RAD18 homolog antibody
  RAD18 S. cerevisiae homolog antibody
  RAD18 S. cerevisiae homolog of antibody
  RAD18 transcript variant antibody
  RAD18_HUMAN antibody
  Rad18sc antibody
  Radiation sensitivity protein 18 antibody
  RING finger protein 73 antibody
  RNF 73 antibody
  RNF73 antibody
  Structural maintenance of chromosomes protein 6 antibody
  YCR066W antibody
  YCR66W antibody
  
• Accession No. Swiss-Prot#:Q9NS91
• UniprotQ9NS91
• Gene ID56852;
• Calculated MW 56/90kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  FC: 1:50-1:100

The RING-type zinc finger protein Rad18 is essential in post-replication repair of damaged DNA and contributes to the maintenance of genomic stability. Rad18 maintains chromosomal DNA with the Rad54-dependent DNA repair pathway and recruits ubiquitin-conjugating enzymes in the Rad6 pathway. Rad18 functions in gap-filling of a daughter strand on replication of damaged DNA and is localized in the nucleus.

• Western blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution. Positive control:
  Lane 1: NIH/3T3
  Lane 2: 293T
  Lane 3: MCF-7
• Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RAD18 antibody. Counter stained with hematoxylin.
• ICC staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining RAD18 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining RAD18 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with RAD18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting