• Product NameAGR2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SN74-01
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, IP, FC
• Species ReactivityHu
• Immunogen Descriptionrecombinant protein
• Alternative Names    AG 2 antibody
  AG 2 protein antibody
  AG-2 antibody
  AG2 antibody
  AG2 protein antibody
  AGR 2 antibody
  AGR2 antibody
  AGR2_HUMAN antibody
  Anterior gradient 2 homolog (Xenopus laevis) antibody
  anterior gradient 2 homolog antibody
  Anterior gradient 2, Xenopus, homolog of antibody
  Anterior gradient homolog 2 antibody
  Anterior gradient protein 2 homolog antibody
  GOB 4 antibody
  GOB4 antibody
  HAG 2 antibody
  hAG-2 antibody
  hAG2 antibody
  HAG2/R antibody
  HPC 8 antibody
  hPC8 antibody
  PDIA17 antibody
  Protein disulfide isomerase family A member 17 antibody
  secreted cement gland homolog antibody
  Secreted cement gland protein XAG 2 homolog antibody
  Secreted cement gland protein XAG-2 homolog antibody
  Secreted cement gland protein XAG2 homolog antibody
  XAG 2 antibody
  XAG2 antibody
  
• Accession No. Swiss-Prot#:O95994
• UniprotO95994
• Gene ID10551;
• Calculated MW 17 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  FC: 1:50-1:100

AGR2 (Anterior Gradient Protein 2), also known as AG2, GOB-4 or HAG-2, is a member of the anterior gradient homolog family. It is the human ortholog of XAG-2, the secreted Xenopus laevis Anterior Gradient protein. In X. laevis, it is involved in cement gland differentiation and neural marker gene expression. AGR2 is a secretory protein encoded by two different AGR2 transcripts. It interacts with LYPD3 and α-dystroglycan (DAG-1). AGR2 is ubiquitously expressed with up-regulated expression in prostate cancer, breast cancer, lung cancer, renal carcinomas and endometrial carcinomas. AGR2 expression is positively correlated with that of the estrogen receptor (ER) and is negatively correlated with that of the epidermal growth factor receptor (EGFR). AGR2 may serve as a potential therapeutic marker for various cancers.

• Western blot analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-AGR2 antibody. Counter stained with hematoxylin.
• ICC staining AGR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining AGR2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining AGR2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with AGR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting