• Product NameHNF-4-alpha Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SN72-03
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu
• Immunogen Descriptionrecombinant protein
• Alternative Names    FLJ39654 antibody
  FRTS4 antibody
  Hepatic nuclear factor 4 alpha antibody
  Hepatocyte nuclear factor 4 alpha antibody
  Hepatocyte nuclear factor 4 antibody
  Hepatocyte nuclear factor 4-alpha antibody
  HNF 4 alpha antibody
  HNF 4 antibody
  HNF-4-alpha antibody
  HNF4 antibody
  HNF4A antibody
  HNF4A_HUMAN antibody
  HNF4a7 antibody
  HNF4a8 antibody
  HNF4a9 antibody
  Hnf4alpha antibody
  HNF4alpha10/11/12 antibody
  MODY 1 antibody
  MODY antibody
  MODY1 antibody
  NR2A1 antibody
  NR2A21 antibody
  Nuclear receptor subfamily 2 group A member 1 antibody
  OTTHUMP00000031060 antibody
  OTTHUMP00000031062 antibody
  TCF 14 antibody
  TCF antibody
  TCF-14 antibody
  TCF14 antibody
  Tcf4 antibody
  Transcription factor 14, hepatic nuclear factor antibody
  Transcription factor 14 antibody
  Transcription factor HNF 4 antibody
  Transcription factor HNF-4 antibody
  Transcription factor HNF4 antibody
  
• Accession No. Swiss-Prot#:P41235
• UniprotP41235
• Gene ID3172;
• Calculated MW 53 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  IHC: 1:100-1:500
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF-4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.

• Western blot analysis of HNF-4-alpha on human kidney lysates using anti-HNF-4-alpha antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.
• ICC staining HNF-4-alpha in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of HepG2 cells with HNF-4-alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting