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Akt2(Phospho-Ser474) Antibody #11124

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Catalog No. Package Size Price Quantity
50ul $219.00
100ul $319.00
Availability:Yes
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Description
  • Product NameAkt2(Phospho-Ser474) Antibody
  • Host SpeciesRabbit
  • ClonalityPolyclonal
  • PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • ApplicationsWB IHC
  • Species ReactivityHu Ms Rt
  • SpecificityThe antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.
  • Immunogen TypePeptide-KLH
  • Immunogen DescriptionPeptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2.
  • Target NameAkt2
  • ModificationPhospho
  • Alternative Names    PKB beta; Protein kinase B; RAC-PK-beta
  • Accession No. Swiss-Prot: P31751
    NCBI Protein: NP _001617.1
  • UniprotP31751
  • Gene ID208;
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Background

Published Papers

The effect of cyclic tensile force on the actin cytoskeleton organization and morphology of human periodontal ligament cells.In Biochem Biophys Res Commun.On 2018 Dec 2 by Wang YF, Zuo ZH et al..PMID: 30401563, , (2018),
PMID: 30401563
Girdin/GIV is upregulated by cyclic tension, propagates mechanical signal transduction, and is required for the cellular proliferation and migration of MG-63 cells.In Biochem Biophys Res Commun.On 2015 Aug 21 by Hu JT, Li Y et al..PMID:26163263, , (2015),
PMID: 26163263
ApoA5 knockdown improves whole-body insulin sensitivity in high-fat-fed mice by reducing ectopic lipid content.In J Lipid Res on 2015 Mar by Jo?o Paulo G Camporez , Shoichi Kanda et al..PMID: 25548259, , (2015),
PMID: 25548259
Targeting steroid receptor coactivator 1 with antisense oligonucleotides increases insulin-stimulated skeletal muscle glucose uptake in chow-fed and high-fat-fed male rats. In Am J Physiol Endocrinol Metab on 2014 Nov 1 by Jennifer L Cantley, Daniel F Vatner,et al..PMID:25159329, , (2014),
PMID: 25159329
View All Published Papers Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987, , (2012),
PMID: 23191987
Please let us know if you have published research using #11124 so that we can cite your reference.

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Application

WBWestern Blotting

IHCImmunohistochemistry

IFImmunofluorescence

ICCImmunocytochemistry

FCFlow Cytometry

IPImmunoprecipitation

EELISA

DBDot Blotting

ChIPChromatin Immunoprecipitation

GICAGold Immunochromatography Assay

NCNegative Control

Species Reactivity

HuHuman

MsMouse 

RtRat 

Dm Drosophila melanogaster

C Caenorhabditis elegans

MkMonkey

RbRabbit

B Bovine 

D Dog

PPig

HmHamster

ChHm Chinese Hamster 

ChkChicken  

ShpSheep