• Product NameCD10 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SN75-07
• PurificationProA affinity purified
• ApplicationsWB, FC, IHC, ICC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    Atriopeptidase antibody
  CALLA antibody
  CD10 antibody
  CD10 antigen antibody
  Common acute lymphocytic leukemia antigen antibody
  DKFZp686O16152 antibody
  EC 3.4.24.11 antibody
  Enkephalinase antibody
  EPN antibody
  Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
  Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
  Membrane metallo endopeptidase antibody
  Membrane metallo endopeptidase variant 1 antibody
  Membrane metallo endopeptidase variant 2 antibody
  Membrane metalloendopeptidase antibody
  Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
  Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
  Membrane metalloendopeptidase variant 1 antibody
  Membrane metalloendopeptidase variant 2 antibody
  MGC126681 antibody
  MGC126707 antibody
  MME antibody
  NEP antibody
  NEP_HUMAN antibody
  Neprilysin antibody
  neprilysin-390 antibody
  neprilysin-411 antibody
  Neutral endopeptidase 24.11 antibody
  Neutral endopeptidase antibody
  Neutral endopeptidase, membrane-associated antibody
  SFE antibody
  Skin fibroblast elastase antibody
  
• Accession No. Swiss-Prot#:P08473
• UniprotP08473
• Gene ID4311;
• Calculated MW 100 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1000
  IHC:1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

CD10, also called the common acute lymphoblastic leukemia antigen (CALLA) and neutral endopeptidase (NEP), is a type II integral membrane glycoprotein. CD10 acts as a zinc metalloprotease that cleaves a variety of biologically active peptides including Angiotensins I and II. It is expressed on early B and T lymphoid precursors, B blasts, some granulocytes, bone marrow stromal cells and certain epithelial cells including some tumor cell lines. CD10 is used as a marker of common acute lymphocytic leukemias and some lymphomas.

• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD10 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD10 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
• ICC staining CD10 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Jurkat cells with CD10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting