• Product NameBRG1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SN20-03
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    ATP dependent helicase SMARCA4 antibody
  ATP-dependent helicase SMARCA4 antibody
  BAF 190 antibody
  BAF190 antibody
  BAF190A antibody
  Brahma protein like 1 antibody
  BRG1 antibody
  BRG1 associated factor 190A antibody
  BRG1 protein antibody
  BRG1-associated factor 190A antibody
  BRM/SWI2 related gene 1 antibody
  Global transcription activator homologous sequence antibody
  global transcription activator snf2l4 antibody
  Homeotic gene regulator antibody
  hSNF2b antibody
  Mitotic growth and transcription activator antibody
  MRD16 antibody
  Nuclear protein GRB1 antibody
  Protein brahma homolog 1 antibody
  Protein BRG-1 antibody
  Protein BRG1 antibody
  RTPS2 antibody
  SMARC A4 antibody
  SMARCA4 antibody
  SMCA4_HUMAN antibody
  SNF2 antibody
  SNF2 beta antibody
  SNF2 like 4 antibody
  SNF2-beta antibody
  SNF2B antibody
  SNF2L4 antibody
  SNF2LB antibody
  Sucrose nonfermenting like 4 antibody
  SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 antibody
  SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody
  SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 antibody
  SWI2 antibody
  Transcription activator BRG1 antibody
  
• Accession No. Swiss-Prot#:P51532
• UniprotP51532
• Gene ID6597;
• Calculated MW 185 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).

• Western blot analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control:
  Lane 1: Jurkat
  Lane 2: HepG2
• Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
• ICC staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting