• Product NameTAPA1/CD81 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.SN206-01
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    26 kDa cell surface protein TAPA 1 antibody
  26 kDa cell surface protein TAPA-1 antibody
  26 kDa cell surface protein TAPA1 antibody
  CD 81 antibody
  CD81 antibody
  CD81 antigen (target of antiproliferative antibody
  1) antibody
  CD81 antigen antibody
  CD81 molecule antibody
  CD81_HUMAN antibody
  CVID6 antibody
  S5.7 antibody
  TAPA 1 antibody
  TAPA1 antibody
  Target of the antiproliferative antibody
  1 antibody
  Tetraspanin 28 antibody
  Tetraspanin-28 antibody
  Tetraspanin28 antibody
  Tspan 28 antibody
  Tspan-28 antibody
  Tspan28 antibody
  
• Accession No. Swiss-Prot#:P60033
• UniprotP60033
• Gene ID975;
• Calculated MW 20 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  FC: 1:50-1:100

CD81, also called TAPA-1, is a type III transmembrane protein that is broadly expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin. CD81 is believed to be involved in both cell growth and signal transduction. It can be present as a multimolecular complex in association with CD37 and/or CD53, or on the surface of B cells in association with CD19, CD21 and/or MHC class II antigens.

• Western blot analysis of CD81 on different lysates using anti-CD81 antibody at 1/1,000 dilution. Positive control:
  Lane 1: JAR
  Lane 2: PC-12
  Lane 3: K562
• Flow cytometric analysis of Jurkat cells with CD81 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
• ICC staining CD81 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD81 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD81 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD81 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD81 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD81 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CD81 antibody. Counter stained with hematoxylin.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting