• Product NameEIF2C3/ Argonaute 3 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JJ201-07
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    5730550L01Rik antibody
  Ago 3 antibody
  Ago3 antibody
  AGO3_HUMAN antibody
  argonaute 3 antibody
  Argonaute3 antibody
  eIF 2C 3 antibody
  eIF-2C 3 antibody
  eIF2C 3 antibody
  Eif2c3 antibody
  EIF2C3 protein antibody
  Eukaryotic translation initiation factor 2C 3 antibody
  Eukaryotic translation initiation factor 2C3 antibody
  FLJ12765 antibody
  hAgo3 antibody
  MGC86946 antibody
  Protein argonaute-3 antibody
  
• Accession No. Swiss-Prot#:Q9H9G7
• UniprotQ9H9G7
• Gene ID192669;
• Calculated MW 97 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  FC: 1:50-1:100

Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).

• Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.
• ICC staining EIF2C3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining EIF2C3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining EIF2C3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining EIF2C3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of N2A cells with EIF2C3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting