• Product NameCD74 Rabbit mAb
• Clone No.JF40-10
• PurificationAffinity-chromatography
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu
• Immunogen DescriptionA synthesized peptide derived from human CD74
• Alternative Names    CD 74 antibody
  CD74 antibody
  CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
  CD74 antigen antibody
  CD74 molecule antibody
  CD74 molecule, major histocompatibility complex, class II invariant chain antibody
  CLIP antibody
  DHLAG antibody
  Gamma chain of class II antigens antibody
  HG2A_HUMAN antibody
  HLA class II histocompatibility antigen gamma chain antibody
  HLA DR antigens associated invariant chain antibody
  HLA DR gamma antibody
  HLA-DR antigens-associated invariant chain antibody
  HLA-DR-gamma antibody
  HLADG antibody
  HLADR antigens associated invariant chain antibody
  Ia antigen associated invariant chain antibody
  Ia antigen-associated invariant chain antibody
  Ia associated invariant chain antibody
  Ia gamma antibody
  Ii antibody
  Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
  la-gamma antibody
  Major histocompatibility complex class II invariant chain antibody
  MHC HLA DR gamma chain antibody
  MHC HLA-DR gamma chain antibody
  p33 antibody
  p35 antibody
  Protein 41 antibody
  
• Accession No. Swiss-Prot#:P04233
• UniprotP04233
• Gene ID972;
• Calculated MW 34 kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

The human histocompatibility leukocyte antigen (HLA) class II-associated invariant chain is composed of at least four polypeptides. One of these polypeptide chains is expressed as a membrane-bound subunit and has been designated CD74. The loading of peptide onto the class II MHC protein (MHC II) appears to be regulated by CD74, which associates with MHC II during its migration to the endosomal compartment, where class II binds pep-tide. CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages. In fact, CD74 is broadly expressed in normal B lymphocytes, regardless of their histocompatibility leukocyte antigen (HLA) phenotype, while a subset of peripheral T lymphocytes that are MHC II negative do not express CD74.

• Western blot analysis of CD74 on Raji cells lysates using anti-CD74 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD74 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD74 antibody. Counter stained with hematoxylin.
• ICC staining CD74 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD74 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD74 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Jurkat cells with CD74 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting