• Product NameARF6 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JF101-2
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    ADP ribosylation factor 6 antibody
  ADP ribosylation factor protein 6 antibody
  ADP-ribosylation factor 6 antibody
  ARF6 antibody
  ARF6_HUMAN antibody
  DKFZp564M0264 antibody
  Small GTP binding protein antibody
  Small GTPase antibody
  
• Accession No. Swiss-Prot#:P62330
• UniprotP62330
• Gene ID382;
• Calculated MW 20 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  
  ICC: 1:100-1:500
  
  

The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of Actin to the Golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization. The human ARF6 gene contains five exons and four introns, and encodes a 175 amino acid protein.

• Western blot analysis of ARF6 on human liver lysates using anti-ARF6 antibody at 1/1,000 dilution.
• ICC staining ARF6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ARF6 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining ARF6 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting