• Product NameNUMB Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM10-023
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    c14_5527 antibody
  C14orf41 antibody
  FLJ31314 antibody
  h Numb antibody
  h-Numb antibody
  Numb antibody
  Numb homolog (Drosophila) antibody
  Numb homolog antibody
  Numb protein homolog antibody
  NUMB_HUMAN antibody
  Protein numb homolog antibody
  Protein S171 antibody
  S171 antibody
  
• Accession No. Swiss-Prot#:P49757
• UniprotP49757
• Gene ID8650;
• Calculated MW 71 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:100
  ICC: 1:50-1:200
  
  

Neuronal cell fate decisions are directed in Drosophila by NUMB, a signaling adapter protein with two protein-protein interaction domains: a phosphotyrosine-binding domain and a proline-rich SH3-binding region (PRR). Mammalian NUMB homologs play a role in the determination of cell fates during development and bind with EPS15, LNX1, and NOTCH1. Conditional mouse mutants with deletion of NUMB in developing sensory ganglia show a reduction in axonal arborization in afferent fibers. Changes in cellular calcium homeostasis influences NUMB-dependent cell fate decisions during development of the nervous system. Chicken NUMB (c-NUMB) protein is localized to the basal cortex of mitotic neuroepithelial cells.

• Western blot analysis of NUMB on A431 cells lysates using anti-NUMB antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NUMB antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using anti-NUMB antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NUMB antibody. Counter stained with hematoxylin.
• ICC staining NUMB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NUMB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NUMB in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting