• Product NameCD59 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM10-71
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, FC,IP
• Species ReactivityHu
• Immunogen Descriptionrecombinant protein
• Alternative Names    16.3A5 antibody
  1F5 antibody
  1F5 antigen antibody
  20 kDa homologous restriction factor antibody
  CD 59 antibody
  CD_antigen=CD59 antibody
  CD59 antibody
  CD59 antigen antibody
  CD59 antigen complement regulatory protein antibody
  CD59 antigen p18 20 antibody
  CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
  CD59 glycoprotein antibody
  CD59 molecule antibody
  CD59 molecule complement regulatory protein antibody
  CD59_HUMAN antibody
  Cd59a antibody
  Complement regulatory protein antibody
  EJ16 antibody
  EJ30 antibody
  EL32 antibody
  FLJ38134 antibody
  FLJ92039 antibody
  G344 antibody
  HRF 20 antibody
  HRF-20 antibody
  HRF20 antibody
  Human leukocyte antigen MIC11 antibody
  Ly 6 like protein antibody
  Lymphocytic antigen CD59/MEM43 antibody
  MAC inhibitory protein antibody
  MAC IP antibody
  MAC-inhibitory protein antibody
  MAC-IP antibody
  MACIF antibody
  MACIP antibody
  MEM43 antibody
  MEM43 antigen antibody
  Membrane attack complex (MAC) inhibition factor antibody
  Membrane attack complex inhibition factor antibody
  Membrane inhibitor of reactive lysis antibody
  MGC2354 antibody
  MIC11 antibody
  MIN1 antibody
  MIN2 antibody
  MIN3 antibody
  MIRL antibody
  MSK21 antibody
  p18 20 antibody
  Protectin antibody
  Surface antigen recognized by monoclonal antibody
  16.3A5 antibody
  T cell activating protein antibody
  
• Accession No. Swiss-Prot#:P13987
• UniprotP13987
• Gene ID966;
• Calculated MW 14/20 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  
  ICC: 1:100-1:500
  IP: 1:10-1:50
  FC: 1:50-1:100

CD59 is a GPI-anchored glycoprotein that is expressed on leukocytes, vascular endothelial cells, various epithelial cells and placenta. CD59 acts together with CD58 in mediating T cell adhesion and activation, and it may be a second ligand of CD2. CD59 functions as a regulator of the terminal pathway of complement by binding to the C8/C9 components of the assembling membrane attack complex (MAC) on host cell membranes, to stop the formation of the lytic pore. CD59 also drives both calcium release and activation of lipid-raft associated signalling molecules such as tyrosine kinases. CD59 gene has two p53-responsive domains that may be implicated in the defense of host cells from damage by the complement system in inflammation, suggesting that p53 could be used to mediate susceptibility of tumor cells to the complement lysis during chemotherapy.

• Western blot analysis of CD59 on different cells lysates using anti-CD59 antibody at 1/500 dilution. Positive control: Lane 1: Human placenta Lane 2: HUVEC Lane 3: K562
• ICC staining CD59 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD59 in JAR cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining CD59 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of NIH-3T3 cells with CD59 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting