• Product NameFKBP12 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM11-09
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    12 kDa FK506-binding protein antibody
  12 kDa FKBP antibody
  Calstabin 1 antibody
  FK506 binding protein 1 antibody
  FK506 binding protein 12 antibody
  FK506 binding protein 1A (12kD) antibody
  FK506 binding protein 1A 12kDa antibody
  FK506 binding protein 1A antibody
  FK506 binding protein T cell 12 kD antibody
  FK506 binding protein, T cell, 12 kD antibody
  FK506 binding protein12 antibody
  FK506-binding protein 1A antibody
  FKB1A_HUMAN antibody
  FKBP 12 antibody
  FKBP 1A antibody
  FKBP-12 antibody
  FKBP-1A antibody
  FKBP1 antibody
  FKBP12 antibody
  FKBP12 Exip3 antibody
  FKBP12C antibody
  fkbp1a antibody
  Immunophilin FKBP12 antibody
  Peptidyl prolyl cis trans isomerase antibody
  Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
  PKC12 antibody
  PKCI2 antibody
  PPIase antibody
  PPIase FKBP1A antibody
  Protein kinase C inhibitor 2 antibody
  Rotamase antibody
  
• Accession No. Swiss-Prot#:P62942
• UniprotP62942
• Gene ID2280;
• Calculated MW 12 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1000
  
  ICC: 1:100-1:500
  
  

Immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases which bind to and mediate the effects of immunosuppressive drugs such as Cyclosporin, FK506 and Rapamycin. FKBP12.6, also known as FK506-binding protein 1B, is a 108 amino acid immunophilin belonging to the FKBP-type PPIase family. Subcellularly localized to the cytoplasm, FKBP12.6 binds to RyR in cardiac muscle sarcoplasmic reticulum and possibly plays a unique physiological role in excitation-contraction coupling in cardiac muscle. FKBP12.6 also catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Ubiquitously expressed, FKBP12.6 is found at highest levels in brain and thymus. FKBP12.6 is expressed as two isoforms produced by alternative splicing.

• Western blot analysis of FKBP12 on different cells lysates using anti-FKBP12 antibody at 1/500 dilution. Positive control:
  Line 1: SH-SY5Y
  Line 2:V937
• ICC staining FKBP12 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining FKBP12 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining FKBP12 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting