• Product NamePABPN1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM11-28
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC, IP
• Species ReactivityHu, Ms
• Immunogen Descriptionrecombinant protein
• Alternative Names    Nuclear poly(A)-binding protein 1 antibody
  OPMD antibody
  PAB2 antibody
  PABII antibody
  PABP 2 antibody
  pABP-2 antibody
  PABP2 antibody
  PABP2_HUMAN antibody
  PABPII antibody
  Pabpn1 antibody
  poly(A) binding protein nuclear 1 antibody
  Poly(A)-binding protein 2 antibody
  Poly(A)-binding protein II antibody
  PolyA binding protein II antibody
  Polyadenylate-binding nuclear protein 1 antibody
  Polyadenylate-binding protein 2 antibody
  
• Accession No. Swiss-Prot#:Q86U42
• UniprotQ86U42
• Gene ID8106;
• Calculated MW 49 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  IP: 1:10-1:50
  FC: 1:50-1:100

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.

• Western blot analysis of PABPN1 on MCF-7 cells lysates using anti-PABPN1 antibody at 1/500 dilution.
• Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PABPN1 antibody. Counter stained with hematoxylin.
• ICC staining PABPN1 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  
  

• ICC staining PABPN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  
  

• Flow cytometric analysis of Hela cells with PABPN1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting