• Product NameTDP43 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM51-10
• PurificationProA affinity purified
• ApplicationsWB, IP, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt, Zebrafish
• Immunogen Descriptionrecombinant protein
• Alternative Names    ALS10 antibody
  OTTHUMP00000002171 antibody
  OTTHUMP00000002172 antibody
  OTTHUMP00000002173 antibody
  TADBP_HUMAN antibody
  TAR DNA binding protein 43 antibody
  TAR DNA binding protein antibody
  TAR DNA-binding protein 43 antibody
  TARDBP antibody
  TDP 43 antibody
  TDP-43 antibody
  TDP43 antibody
  
• Accession No. Swiss-Prot#:Q13148
• UniprotQ13148
• Gene ID23435;
• Calculated MW 45 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  IP: 1:10-1:50
  FC: 1:50-1:100

DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.

• Western blot analysis of TDP43 on different cells lysates using anti-TDP43 antibody at 1/500 dilution. Positive control��
  Line 1: Hela
  Line 2: K563
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP43 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TDP43 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TDP43 antibody. Counter stained with hematoxylin.
• ICC staining TDP43 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining TDP43 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining TDP43 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with TDP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting