• Product NameNMDAR1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM11-26
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    GluN1 antibody
  Glutamate [NMDA] receptor subunit zeta-1 antibody
  Glutamate receptor ionotropic N methyl D aspartate 1 antibody
  Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
  glutamate receptor ionotropic, NMDA 1 antibody
  Grin1 antibody
  MRD8 antibody
  N methyl D aspartate receptor antibody
  N methyl D aspartate receptor channel subunit zeta 1 antibody
  N methyl D aspartate receptor subunit NR1 antibody
  N-methyl-D-aspartate receptor subunit NR1 antibody
  NMD-R1 antibody
  NMDA 1 antibody
  NMDA R1 antibody
  NMDA receptor 1 antibody
  NMDA1 antibody
  NMDAR antibody
  NMDZ1_HUMAN antibody
  NR1 antibody
  
• Accession No. Swiss-Prot#:Q05586
• UniprotQ05586
• Gene ID2902;
• Calculated MW 105 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:100-1:500
  
  FC: 1:50-1:100

NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

• Western blot analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control��
  Line 1: MCF-7
  Line 2: A549
• Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin.
• ICC staining NMDAR1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NMDAR1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NMDAR1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of SH-SY5Y cells with NMDAR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting