• Product NamehnRNP A1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JA39-21
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu, Ms,Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    HNRNPA 1 antibody
  Helix destabilizing protein antibody
  Helix-destabilizing protein antibody
  Heterogeneous nuclear ribonucleoprotein A1 antibody
  Heterogeneous nuclear ribonucleoprotein A1B protein antibody
  Heterogeneous nuclear ribonucleoprotein B2 protein antibody
  Heterogeneous nuclear ribonucleoprotein core protein A1 antibody
  hnRNP A1 antibody
  hnRNP core protein A1 antibody
  HNRNPA1 antibody
  HNRPA1 antibody
  MGC102835 antibody
  Nuclear ribonucleoprotein particle A1 protein antibody
  ROA1_HUMAN antibody
  Single strand DNA binding protein UP1 antibody
  Single strand RNA binding protein antibody
  Single-strand RNA-binding protein antibody
  
• Accession No. Swiss-Prot#:P09651
• UniprotP09651
• Gene ID3178;
• Calculated MW 33 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  
  ICC: 1:50-1:200
  
  

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. The A/B subfamily of hnRNPs include A1, A2/B1, A3 and A0, and in Xenopus, hnRNP A1, A2 and A3 are ubiquitously expressed throughout development as well as in adult tissues. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The hnRNP A0 gene is distinct from the other A/B family members, and it encodes a low-abundance protein, which is implicated in mRNA stability.

• Western blot analysis of hnRNP A1 on HepG2 (1) and PC-12 (2) cell using anti-hnRNP A1 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-hnRNP A1 antibody. Counter stained with hematoxylin.
• ICC staining hnRNP A1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining hnRNP A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining hnRNP A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Jurkat cells with hnRNP A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting