• Product NameCalpain 2 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JA43-41
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    Calcium activated neutral proteinase 2 antibody
  Calcium activated neutral proteinase antibody
  Calcium-activated neutral protease 2, catalytic subunit antibody
  Calcium-activated neutral proteinase 2 antibody
  CALP80 antibody
  Calpain 2 (m/II) large subunit antibody
  Calpain 2 catalytic subunit antibody
  Calpain 2 large catalytic subunit antibody
  Calpain 2 large subunit antibody
  Calpain 2, large [catalytic] subunit antibody
  Calpain large polypeptide L2 antibody
  Calpain M type antibody
  Calpain M-type antibody
  Calpain-2 catalytic subunit antibody
  Calpain-2 large subunit antibody
  Calpain2 antibody
  CAN2_HUMAN antibody
  CANP 2 antibody
  CANP L2 antibody
  CANP2 antibody
  CANPL 2 antibody
  CANPL2 antibody
  CANPml antibody
  Capa2 antibody
  CAPN 2 antibody
  CAPN2 antibody
  FLJ39928 antibody
  M calpain antibody
  M calpin antibody
  M type antibody
  M-calpain antibody
  mCANP antibody
  Millimolar calpain antibody
  Millimolar-calpain antibody
  
• Accession No. Swiss-Prot#:P17655
• UniprotP17655
• Gene ID824;
• Calculated MW 80 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  
  
  

Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, is thought to play a role in cytoskeletal reorganization and muscle protein degradation. Calpain exists as a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.

• Western blot analysis of Calpain 2 on Hela cell (1) and rat skin tissue (2) lysate using anti-Calpain 2 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.

• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 2 antibody. Counter stained with hematoxylin.

• ICC staining Calpain 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with Calpain 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting