• Product NameEphrin B2 Rabbit mAb
• Clone No.JM53-21
• PurificationAffinity-chromatography
• ApplicationsWB, ICC/IF
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionA synthesized peptide derived from human Ephrin B2
• Alternative Names    EFN B2 antibody
  EFNB 2 antibody
  Efnb2 antibody
  EFNB2_HUMAN antibody
  Eph related receptor tyrosine kinase ligand 5 antibody
  EPH-related receptor tyrosine kinase ligand 5 antibody
  ephrin B2 antibody
  Ephrin-B2 antibody
  EphrinB2 antibody
  EPLG 5 antibody
  EPLG5 antibody
  Htk L antibody
  HTK ligand antibody
  HTK-L antibody
  HTKL antibody
  LERK 5 antibody
  LERK-5 antibody
  LERK5 antibody
  Ligand of eph related kinase 5 antibody
  MGC126226 antibody
  MGC126227 antibody
  MGC126228 antibody
  OTTMUSP00000024973 antibody
  
• Accession No. Swiss-Prot#:P52799
• UniprotP52799
• Gene ID1948;
• Calculated MW 50 kDa
• Formulation Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

• WB 1:1000-1:2000  ICC/IF 1:50-1:200

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.

• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
• Western blot analysis of Ephrin B2 expression in (1) HEK293 cell lysate; (2) Mouse spleen lysate.
• Immunofluorescent analysis using the Antibody at 1:50 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting