• Product NameSkp1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM27-37
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, FC
• Species ReactivityHu, Ms, Rt
• Immunogen Descriptionrecombinant protein
• Alternative Names    Cyclin A/CDK2 associated p19 antibody
  Cyclin A/CDK2 associated protein p19 antibody
  Cyclin-A/CDK2-associated protein p19 antibody
  EMC19 antibody
  MGC34403 antibody
  OCP 2 antibody
  OCP II antibody
  OCP II protein antibody
  OCP-2 antibody
  OCP-II antibody
  OCP2 antibody
  OCPII antibody
  Organ of Corti protein 2 antibody
  Organ of Corti protein II antibody
  OTTHUMP00000159379 antibody
  OTTHUMP00000159380 antibody
  OTTHUMP00000228275 antibody
  OTTHUMP00000228276 antibody
  OTTHUMP00000228278 antibody
  OTTHUMP00000228279 antibody
  OTTHUMP00000228281 antibody
  p19A antibody
  p19skp1 antibody
  RNA polymerase II elongation factor like protein antibody
  RNA polymerase II elongation factor like protein OCP2 antibody
  RNA polymerase II elongation factor-like protein antibody
  S phase kinase associated protein 1 antibody
  S phase kinase associated protein 1A antibody
  S phase kinase associated protein 1A p19A antibody
  S-phase kinase-associated protein 1 antibody
  SIII antibody
  Skp 1 antibody
  SKP 1A antibody
  skp1 antibody
  SKP1_HUMAN antibody
  SKP1A antibody
  TCEB1L antibody
  Transcription Elongation Factor B 1 like antibody
  Transcription elongation factor B antibody
  Transcription elongation factor B SIII polypeptide 1 like antibody
  
• Accession No. Swiss-Prot#:P63208
• UniprotP63208
• Gene ID6500;
• Calculated MW 19 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500
  
  ICC: 1:50-1:100
  
  FC: 1:50-1:100

Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

• Western blot analysis of Skp1 on PC-12 cell using anti-Skp1 antibody at 1/1,000 dilution.
• ICC staining Skp1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Skp1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with Skp1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting