• Product NameNFkB p100 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JM82-03
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    CVID10 antibody
  DNA binding factor KBF2 antibody
  H2TF1 antibody
  Lymphocyte translocation chromosome 10 protein antibody
  LYT 10 antibody
  NF kB2 antibody
  NFKB p52/p100 subunit antibody
  Nuclear factor Kappa B subunit 2 antibody
  Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 (p49/p100) antibody
  Nuclear factor of kappa light polypeptide gene enhancer in B cells 2 antibody
  Oncogene Lyt 10 antibody
  p100 antibody
  Transcription factor NFKB2 antibody
  
• Accession No. Swiss-Prot#:Q00653
• UniprotQ00653
• Gene ID4791;
• Calculated MW 100 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  

NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

• Western blot analysis of NFkB p100 on Hela (1) and MCF-7 (2) cell using anti-NFkB p100 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFkB p100 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NFkB p100 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFkB p100 antibody. Counter stained with hematoxylin.
• ICC staining NFkB p100 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining NFkB p100 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting