• Product NamemtTFA Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, IHC, IP
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein
• Alternative Names    anscription factor 6-like 1 antibody
  Mitochondrial transcription factor 1 antibody
  mitochondrial transcription factor A antibody
  MtTF1 antibody
  mtTFA antibody
  TCF 6 antibody
  TCF-6 antibody
  TCF6 antibody
  TCF6L1 antibody
  TCF6L2 antibody
  TCF6L3 antibody
  TFAM antibody
  TFAM_HUMAN antibody
  Transcription factor 6 antibody
  Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
  Transcription factor 6 like 2 antibody
  Transcription factor 6-like 2 antibody
  transcription factor 6-like 3 antibody
  Transcription factor A, mitochondrial antibody
  Transcription factor A, mitochondrial antibody
  Transcription factor A, mitochondrial precursor antibody
  
• Accession No. Swiss-Prot#:Q00059
• UniprotQ00059
• Gene ID7019;
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:200-1:500
  IHC: 1:50-1:200
  ICC/IF: 1:50-1:200
  IP: 1:10-1:50
  

Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.

• Western blot analysis of mtTFA on Hela cell using anti-mtTFA antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-mtTFA antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-mtTFA antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-mtTFA antibody. Counter stained with hematoxylin.
• ICC staining mtTFA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining mtTFA in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining mtTFA in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting