• Product NameRANTES Rabbit mAb
• Clone No.JM03-45
• PurificationProA affinity purified
• ApplicationsWB,ICC,IF,IHC
• Species ReactivityHu, Ms
• Immunogen DescriptionRecombinant protein
• Alternative Names    Beta chemokine RANTES antibody
  Beta chemokine RANTES precursor antibody
  C C motif chemokine 5 antibody
  CCL 5 antibody
  CCL5 antibody
  CCL5_HUMAN antibody
  Chemokine (C C motif) ligand 5 antibody
  Chemokine CC Motif Ligand 5 antibody
  D17S136E antibody
  EoCP antibody
  Eosinophil chemotactic cytokine antibody
  MGC17164 antibody
  RANTES(4-68) antibody
  Regulated upon activation normally T expressed and presumably secreted antibody
  SCYA 5 antibody
  SCYA5 antibody
  SIS delta antibody
  SIS-delta antibody
  SISd antibody
  Small inducible cytokine A5 (RANTES) antibody
  Small inducible cytokine A5 antibody
  Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
  Small-inducible cytokine A5 antibody
  T cell specific protein p288 antibody
  T cell specific RANTES protein antibody
  T cell-specific protein P228 antibody
  T-cell-specific protein RANTES antibody
  TCP 228 antibody
  TCP228 antibody
  
• Accession No. Swiss-Prot#:P13501
• UniprotP13501
• Gene ID6352;
• Calculated MW 10 kDa
• Concentration 1 mg/ml
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:1,000
  IHC: 1:50-1:200
  ICC/IF: 1:50-1:200

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.

• Western blot analysis of RANTES on mouse thymus tissue lysate using anti-RANTES antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RANTES antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-RANTES antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human lung caner tissue using anti-RANTES antibody. Counter stained with hematoxylin.
• ICC staining RANTES in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining RANTES in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting