• Product NamePSMA1 Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JU66-24
• PurificationProA affinity purified
• ApplicationsWB,ICC/IF,IHC,IP,FC
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    30 kDa prosomal protein antibody
  HC 2 antibody
  HC2 antibody
  Macropain subunit C2 antibody
  Macropain subunit nu antibody
  MGC14542 antibody
  MGC14575 antibody
  MGC14751 antibody
  MGC1667 antibody
  MGC21459 antibody
  MGC22853 antibody
  MGC23915 antibody
  Multicatalytic endopeptidase complex subunit C2 antibody
  NU antibody
  PROS 30 antibody
  PROS-30 antibody
  PROS30 antibody
  Proteasome (prosome macropain) subunit alpha type 1 antibody
  Proteasome alpha 1 subunit antibody
  Proteasome component C2 antibody
  Proteasome nu chain antibody
  Proteasome subunit alpha type 1 antibody
  Proteasome subunit alpha type I antibody
  Proteasome subunit alpha type-1 antibody
  Proteasome subunit nu antibody
  Protein P30 33K antibody
  PSA1_HUMAN antibody
  PSC 2 antibody
  PSC2 antibody
  PSMA 1 antibody
  psmA1 antibody
  
• Accession No. Swiss-Prot#:P25786
• UniprotP25786
• Gene ID5682;
• Calculated MW 30 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:200-1:1,000
  
  FC: 1:50-1:100

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome. Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.

• Western blot analysis of PSMA1 on different cell lysates using anti-PSMA1 antibody at 1/500 dilution. Positive control:
  Lane 1: Jurkat
  Lane 2: Hela
  Lane 3: HepG2
  Lane 4: 293
  
• Western blot analysis of PSMA1 on different lysates using anti-PSMA1 antibody at 1/500 dilution. Positive control:
  Lane 1: PC-12
  Lane 2: Mouse spleen tissue
  Lane 3: Rat spleen tissue
  
• Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.
• ICC staining PSMA1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PSMA1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining PSMA1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting