• Product NameApg3 Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.JU43-26
• PurificationProA affinity purified
• ApplicationsWB,ICC/IF,IHC,IP
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Accession No. Swiss-Prot#:P68431
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• Calculated MW 11/15 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:200-1:1,000
  IP: 1:10-1:50
  

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

• Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.
• ICC staining Histone H3 (acetyl K14) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Histone H3 (acetyl K14) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Histone H3 (acetyl K14) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting