• Product NameSDHB Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.JU02-33
• PurificationProA affinity purified
• ApplicationsWB,IHC,FC,IP
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein
• Alternative Names    CWS2 antibody
  DHSB_HUMAN antibody
  FLJ92337 antibody Ip antibody
  Iron sulfur subunit antibody
  Iron sulfur subunit of complex II antibody
  Iron-sulfur subunit of complex II antibody
  mitochondrial antibody
  PGL 4 antibody
  PGL4 antibody
  SDH 1 antibody
  SDH antibody
  SDH1 antibody
  SDH2 antibody
  SDH2, homolog of antibody
  SdhB antibody
  SDHIP antibody
  Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody
  Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody
  Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody
  succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody
  Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody
  Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody
  Succinate dehydrogenase complex iron sulfur subunit B antibody
  Succinate dehydrogenase complex subunit B iron sulfur antibody
  Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody
  succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody
  Succinate dehydrogenase iron sulfur protein antibody
  
• Accession No. Swiss-Prot#:P21912
• UniprotP21912
• Gene ID6390;
• Calculated MW 32 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  
  IP: 1:10-1:50
  FC: 1:50-1:100

In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.

• Western blot analysis of SDHB on different lysates using anti-SDHB antibody at 1/500 dilution.
  Positive control:
  Lane 1: Human liver
  Lane 2: Rat spleen
  Lane 3: Rat liver
  Lane 4: Mouse spleen
  Lane 5: HepG2
  
• Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-SDHB antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SDHB antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-SDHB antibody. Counter stained with hematoxylin.
• Flow cytometric analysis of Jurkat cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting